Fig. 3

Inhibition of mitolysosome formation by QA induces microglial senescence. (A) Representative images of BV2 cells stained with SA-β-Gal treated with or without 50 µM QA for 72 h. Scale Bar, 50 μm. (C) Relative mRNA level of Glb1 in BV2 cells treated with or without 50 µM QA for 72 h (n = 3). (D) Relative mRNA levels of p16, p21 and p53 in BV2 cells treated with or without 50 µM QA for 72 h (n = 2–4). (E) Representative images of BV2 cells stained with SA-β-Gal treated with or without 50 µM QA in the absence or presence of 10 µM UA for 72 h. Scale Bar, 50 μm. (B, F) Graphs represent percent of SA-β-Gal positive cells (green staining) per imaging field. Data are means ± SEM (B) n = 5–6 imaging fields and (F) n = 3 imaging fields; * p < 0.05, ** p < 0.01 and *** p < 0.001 versus no treatment (control) group, ^###p < 0.001 versus QA alone treatment group; One-way ANOVA followed by Tukey’s multiple comparisons test (B and F) or two-tailed unpaired t test (C, D)