Fig. 4

Overregulated miR-338-3p targeting THBS1 attenuated glioma cell growth by suppressing the PI3K/Akt pathway. (A) The western blot analysis detected THBS1 levels in U251 and A172 cells transfected with mimic-NC, empty vector, THBS1-OE (OE), miR-338-3p mimic (mimic), or OE + mimic. (B, C) Proliferation (B) and migration (C) of U251 and A172 cells transfected with mimic-NC, empty vector, OE, mimic, or OE + mimic were evaluated by performing CCK-8 and wound-healing analyses. (D) Levels of phosphorylated AKT and PI3K proteins in U251 and A172 cells harboring mimic-NC, empty vector, OE, mimic, or OE + mimic were assessed by performing western blot analysis. ^*P < 0.05, ^**P < 0.001 vs. Empty vector; ^#P < 0.05, ^##P < 0.001 vs. mimic-NC; ^&P < 0.05, ^&&P < 0.001 vs. OE + mimic.