Fig. 1

miR-338-3p was downregulated in glioma, and miR-338-3p overregulation repressed the migration and proliferation of tumor cells by suppressing PI3K/Akt phosphorylation. (A) miR-338-3p expression was assessed in normal and glioma samples by performing qPCR. (B) miR-338-3p levels were assessed in primary normal human astrocytes (NHA) and glioma cells (HS683, U251, and A172) via qPCR. ^**P < 0.001 vs. NHA. (C) miR-338-3p expression was determined in U251 and A172 cells harboring either mimic-NC (NC) or miR-338-3p mimic (mimic). ^**P < 0.001 vs. NC. (D, E) CCK-8 (D) and wound-healing (E) assays were performed in U251 and A172 cells with NC or mimic transfection to assess the abilities of the cells to proliferate and migrate. ^**P < 0.001 vs. NC. (F) Levels of phosphorylated PI3K and AKT proteins in U251 and A172 cells with NC or miR-338-3p transfection were determined by performing western blotting. ^**P < 0.001 vs. NC