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Fig. 2 | Biology Direct

Fig. 2

From: RCC2 promotes prostate cancer cell proliferation and migration through Hh/GLI1 signaling pathway and cancer stem-like cells

Fig. 2

RCC2 promotes cell propagation of prostate cancer. A The protein levels of RCC2 in two prostate cancer cell lines (DU145 and PC3) and a normal prostate cell line (RWPE-2) were measured using western blot. B The protein levels of RCC2 in DU145 and PC3 cells transfected with siRCC2#1, siRCC2#2, and siCtrl for 48 h were decided with western blot. C Cell viability of DU145 and PC3 cells transfected for 24, 48, and 72 h were evaluated using CCK-8 assays. D Cell colony generation assays of DU145 and PC3 cells transfected for two weeks. E The protein level of RCC2 in DU145, PC3 and RWPE-2 cells transfected with pcDNA-RCC2 and pcDNA-ctrl for 48 h were decided with western blot. F Cell viability was assessed using CCK-8 assays. G and H Cell colony generation ability was detected using cell colony formation assays. *P＜0.05, ***P＜0.001

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