Fig. 5

Overexpression of ACSM5 improved LF hypertrophy by inhibiting FABP4-mediated PPAR signaling pathway in mice. (A) Western blot was used to detect the protein expression of ACSM5, FABP4, and PPARγ in LF hypertrophy mice treated with AAV-ACSM5 alone or in combination with AAV-FABP4. (B and C) After ADV-ACSM5 alone or in combination with ADV-FABP4 treatment of mice with LF hypertrophy, the LF area was assessed using HE staining. Bar size = 50 nm. (D) After ADV-ACSM5 alone or in combination with ADV-FABP4 treatment of mice with LF hypertrophy, and TG assay kit was used to detect TG levels in LF tissue. (E and F) After ADV-ACSM5 alone or in combination with ADV-FABP4 treatment of mice with LF hypertrophy, and the protein expression of FASN and ACC in LF tissue was detected by western blot. (E and H) After ADV-ACSM5 alone or in combination with ADV-FABP4 treatment of mice with LF hypertrophy, and the protein expression of collagen I, collagen III, and α-SMA in LF tissue was detected by western blot. LF, ligamentum flavum. Model, mice with LF hypertrophy. AAV, adeno-associated virus. NC, negative control. ^n.s.P > 0.05, ^#P < 0.05, ^##P < 0.01, and ^###P < 0.001