Fig. 8

MiR-31-5p regulated the neuroinflammatory response via TRAF6 in CCI mice models. Neuron 2a cells were transfected with inhr (miR-31-5p inhibitor) or Scr(scramble) for 48 h. a, b Western blotting analysis of TRAF6 protein expression in five groups. ^** P < 0.01 vs. naive; ^## P < 0.01 vs. inhr. two-way ANOVA, n = 3. c RT-qPCR analysis of miR-31-5p expression in five groups. ^** P < 0.01 vs. naive; two-way ANOVA, n = 3. RT-qPCR analysis of TNF‐α (d), IL‐6 (e) and IL‐1β (f) expression in five groups.^*P < 0.05 vs. naive ^** P < 0.01 vs. naive; ^## P < 0.01 vs. inhr. two-way ANOVA, n = 6. DRG microinjection of miR-31-5p^−/− mice received Scr or TRAF6 siRNA. RT-qPCR analysis of TNF‐α (g), IL‐6 (h) and IL‐1β (i) expression in the DRG of miR-31^−/− mice injected with Scr or TRAF6 siRNA. ^* P < 0.05, ^** P < 0.01, two- tailed unpaired t-test, n = 6 mice. j Mechanical withdrawal threshold in miR-31-5p^−/− mice with TRAF6 siRNA, ^** P < 0.01. two-way ANOVA, n = 8 mice. k Heat withdrawal latency in miR-31-5p^−/− mice with TRAF6 siRNA, ^* P < 0.05 vs. miR-31^−/− + Scr, ^** P < 0.01 vs. miR-31^−/− + Scr. two-way ANOVA, n = 8 mice