Figure. 6

TRAF6 could relieve neuropathic pain a The sequence of miR‐31-5p and the position of TRAF6 3′‐UTR. b, c Western blotting analysis of TRAF6 on days 0, 3, 7 and 14 after CCI surgery. ^*P < 0.05 vs. sham. ^**P < 0.01 vs. sham, one -way ANOVA, n = 3. d RT-qPCR analysis of TRAF6 on days 0, 3, 7 and 14 after CCI surgery. ^**P < 0.01 vs. sham, one -way ANOVA, n = 3. e, f Fluorescence images of TRAF6 (red), NeuN(green) and DAPI (blue) were obtained in DRGs of sham and CCI mice, The scale bar is 50μm, ^**P < 0.01 vs. sham, Two-tailed unpaired t-test, n = 3. g, h Western blotting analysis of TRAF6 in the neuron 2a cells with TRAF6 siRNA or scramble transfection. ^** P < 0.01 vs. scramble. Two- tailed unpaired t-test, n = 3. DRG microinjection of sham and CCI mice received Scr or TRAF6 siRNA. i Mechanical withdrawal threshold in four groups, ^**P < 0.01 vs. sham+ Scr. ^##P < 0.01 vs. CCI+ Scr, two-way ANOVA, n = 8 mice. j Heat withdrawal latency in four groups, ^**P < 0.01 vs. sham+ Scr, ^#P < 0.05 vs. CCI+ Scr, ^##P < 0.01 vs. CCI+ Scr, two-way ANOVA, n = 8 mice.