Figure. 5

Intrathecal miR-31-5p mimic attenuated neuropathic pain. a The description of the animal experimental process. Behavior tests were performed on postoperative days and after scramble or miR-31-5p mimic administration. b Relative expression of miR-31-5p in the DRG of CCI mice treated with intrathecal miR-31-5p mimics/scrambles was determined by qRT-PCR. ^**P < 0.01 vs. sham + scramble; ^##P < 0.01 vs. CCI + scramble. one-way ANOVA, n = 7 mice. c Effects of the upregulated miR-31-5p on mechanical allodynia were assessed by withdrawal threshold on post-induction days 5, 7, 9, and 14. ^**P < 0.01 vs. sham + scramble; ^##P < 0.01 vs. CCI + scramble. Two-way ANOVA, n = 8 mice. d Effects of upregulated miR-31-5p on thermal hyperalgesia were assessed by heat withdrawal latency on post-induction days 5, 7, 9, and 14. ^** P < 0.01 vs. sham + scramble; ^##P < 0.01 vs. CCI + scramble. Two-way ANOVA, n = 8 mice. RT-qPCR analysis of TNF‐α (e), IL‐6 (f) and IL‐1β (g) expression in the DRG of CCI mice injected with Scr or mimic. ^**P < 0.01, two- tailed unpaired t-test, n = 6 mice.