Figure. 4

Downregulated miR-31-5p levels in the CCI mouse model. a The CCI model was established to induce neuropathic pain in mice. b Mechanical withdrawal threshold in sham and CCI mice on postoperative day 0, 3, 7, 10, and 14, ^*P < 0.05, ^**P < 0.01 vs. sham. Two-way ANOVA, n = 8 mice. c Heat withdrawal latency in sham and CCI mice on postoperative day 0, 3, 7, 10, and 14.^*P < 0.05, ^**P < 0.01 vs. sham. Two-way ANOVA, n = 8 mice. d RT-qPCR analysis of miR-31-5p expression in the DRG of CCI mice at the different time points, ^* P < 0.05, ^**P < 0.01 vs. sham. Two-way ANOVA, n = 6 mice. RT-qPCR analysis of TNF‐α(E), IL‐6(F) and IL‐1β(G) expression in the DRG of sham and CCI mice. ^**P < 0.01, two- tailed unpaired t-test, n = 6 mice. CCI, chronic constriction injury; ANOVA, analysis of variance; qRT-PCR: quantitative reverse transcription‐polymerase chain reaction; DRG, dorsal root ganglion.