Figure. 2

MiPEP31 administration could lead to a decrease in murine pain threshold. a–f Mice received Scr(scPEP) or PEP31(miPEP31) (50ug in 100 µL phosphate-buffered saline) via tail vein injections. a Relative expression of miR-31-5p in the DRG of mice on day 3 after injection. ^*P < 0.05 vs. naive. One-way ANOVA, n = 7 mice. b Mechanical withdrawal threshold in three groups, ^* P < 0.05, ^** P < 0.01 vs. naive. two-way ANOVA, n = 8 mice. c Heat withdrawal latency in three groups, ^*P < 0.05, ^**P < 0.01 vs. naive. Two-way ANOVA, n = 8 mice. RT-qPCR analysis of TNF‐α (d), IL‐6 (e) and IL‐1β (f) expression in the DRG of mice injected with miPEP31after 3 days. ^**P < 0.01, two- tailed unpaired t-test, n = 6 mice.