Fig. 5
From: LncRNA WAC-AS1 promotes osteosarcoma Metastasis and stemness by sponging miR-5047 to upregulate SOX2
 A WAC-AS1/SOX2 feedback loop enhances OS malignancy. A. JASPAR was utilized to predict the possible binding sites of transcription factor SOX2 in the WAC-AS1 promoter region. B. WAC-AS1 expression was determined, by qRT-PCR, after SOX2 transfection or knockdown on in OS cells. C. ChIP was used to examine the binding of SOX2 with the WAC-AS1 promoter at two predicted sites (R1 and R2). D. Schematic diagram of luciferase reporter vectors containing a wild-type or mutant R2 sequence in the WAC-AS1 promoter. Dual luciferase reporter assay was performed in OS cells transfected with the wild-type or mutant reporter. E. Colony formation of sh-WAC-AS1-transfected, SOX2-transfected or control OS cells. F. Flow cytometry detecting apoptosis of sh-WAC-AS1-transfected, SOX2-transfected or control OS cells. G. Transwell assay showing the migration of sh-WAC-AS1-transfected, SOX2-transfected or control OS cells(scale bar, 50 μm). H. Wound healing assay showing the migration of sh-WAC-AS1-transfected, SOX2-transfected or control OS cells(scale bar, 100 μm). I. Schematic diagram of this project. Error bars represent three independent experiments, *p < 0.05, **p < 0.01, *** p < 0.001