Fig. 2
From: LncRNA WAC-AS1 promotes osteosarcoma Metastasis and stemness by sponging miR-5047 to upregulate SOX2
Determination of WAC-AS1 function in OSin vivo and in vitro. A. CCK8 assay measuring the proliferation of WAC-AS1-transfected or control (NC) OS cells. B. Colony formation by WAC-AS1-transfected or control OS cells. C. Flow cytometry detecting apoptosis of WAC-AS1-transfected or control OS cells. D.PARP and cleaved PARP (cl-PARP), Bcl-2 and Bax were detected by western blotting in WAC-AS1 and control OS cells. E. Wound healing assay showing the migration of WAC-AS1-transfected or control OS cells (scale bar, 100 μm). F. Transwell assay showing the migration of WAC-AS1-transfected or control OS cells (scale bar, 50 μm). G. Representative images of xenograft tumors (n = 6) resulting from WAC-AS1-overexpressing and control cells. Tumor growth curves and tumor weight are shown. H. H&E staining and IHC staining of caspase-3 and Ki67 in control and WAC-AS1-overexpressing tumors (scale bar, 100 μm). I. Lung metastases (n = 5) following tail vein injection of WAC-AS1-overexpressing and control cells (scale bar, 100 μm). Error bars represent three independent experiments, *, p < 0.05, **p < 0.01, *** p < 0.001