Fig. 1

Expression of WAC-AS1 in OS and its clinical significance. A. Heatmap of differentially-expressed lncRNAs in cancer stem cells (CSCs) and control cells is shown based on RNA-seq data. Venn diagram displaying 28 differentially-expressed lncRNAs in CSCs vs. parental OS cells for both MG62 and U2OS cell lines. B. Expression of WAC-AS1 was detected in parental MG63 and U2OS osteosarcoma cells and CSCs. C. WAC-AS1 expression was higher in cancer tissues than normal tissues, based on TCGA analysis. D. Expression of WAC-AS1 in the cytoplasm and nucleus of OS cells as determined by qRT-PCR. E. Location of WAC-AS1 was detected by fluorescence in situ hybridization (FISH) (scale bar: 20 μm). F. Expression of WAC-AS1 was detected by qRT-PCR in OS tissues (n = 20) and normal tissues (n = 5). G. Expression of WAC-AS1 was detected by ISH in 72 OS cases and 6 normal tissues (scale bar: 100 μm, 25 μm). H. Expression of WAC-AS1 was detected by ISH in tumors from 63 OS non-metastasis cases and 9 metastasis cases (scale bar: 100 μm, 25 μm). I. Survival curve of OS patients with high and low WAC-AS1 expression is shown. Error bars represent three independent experiments, *, p < 0.05, **p < 0.01, *** p < 0.001