Fig. 6

RNA-seq analysis of mRNA profiling in cEVs and nEVs. (A) Pearson’s correlation analysis showing a high degree of similarity between the mRNA expression profiles for the cEVs (n = 3) and nEVs (n = 3) samples. (B) Volcano plot showing differently expressed mRNAs between cEVs (n = 3) and nEVs (n = 3) samples. Red dot represented differently expressed mRNAs with P < 0.05 and |Log2FC|>1, green dot represented mRNAs with P > 0.05 and |Log2FC|>1, blue dot represented mRNAs with P < 0.05 and |Log2FC|<1, grey dot represented mRNAs with not significant. C, D. Gene Ontology (GO) analysis of the overexpressed mRNAs in cEVs (C) or nEVs (D), including biological process (BP), cellular component (CC), and molecular function (MF). E, F. KEGG analysis of the overexpressed mRNAs in cEVs (E) or nEVs (F). NOTEs: mmu01100: metabolic pathways; mmu04530: tight junction; mmu00190: oxidative phosphorylation; mmu05208: chemical carcinogenesis - reactive oxygen species; mmu00561: glycerolipid metabolism; mmu04662: B cell receptor signaling pathway; mmu04611: platelet activation; mmu04670: leukocyte transendothelial migration; mmu04064: NF-kappa B signaling pathway; mmu04660: T cell receptor signaling pathway