Fig. 5

cEVs and nEVs treatment promoted angiogenesis after MI. (A) Representative images of CD31 staining in myocardial sections at 28 days from MI mice treated with PBS, cEVs, or nEVs (scale bar = 50 μm, n = 6). (B) Quantitative myocardial capillary density in mice hearts at day 28 post-MI. C-F. Relative mRNA expressions of VEGF-A (C), VEGF-B (D), Kdr (E), and Pecam (F) in PBS, cEVs, or nEVs-treated mice hearts at day 28 after MI (n = 3). G. Representative images showing the HUVEC-formed tube structure in different groups (scale bar = 200 μm, n = 5). H-J. Analysis of total tube length (H), number of branches (I), and number of junctions (J) formed by HUVECs in different groups. (*P < 0.05, **P < 0.01, ***P < 0.001, ns, not significant)