Fig. 4

Administration of both cEVs and nEVs alleviated cardiomyocytes apoptosis after MI. (A) Representative images of Tunel staining in myocardial sections at 3 days from MI mice treated with PBS, cEVs, or nEVs (scale bar = 50 μm, n = 6). (B) Analysis of Tunel positive cells (cell counts per field) in the cardiac immunofluorescence images. C-E. Protein analysis of Bax, Bcl-2, Cleaved-Caspase 3, and Total-Caspase 3 in mice treated with PBS, cEVs, or nEVs (n = 3). F, G. Apoptosis rate of cardiomyocytes with different treatments detected by flow cytometry (F) and representative analysis (G) (n = 4). (*P < 0.05, **P < 0.01, ***P < 0.001, ns, not significant)