Fig. 5

Reduction of I_Ca,L in Cs⁺ _in/Cs⁺ _out solutions with K⁺ currents suppressed during local stretching of cardiomyocytes by 6, 8, and 10 μm. V_hp = − 45 mV. A: (6 μm stretch). A.1 – The time course of the membrane current. The holding current at V_hp in control (beginning of the blue traces - label C) and during stretching (beginning of the red traces – label S). A pulse from − 45 to 0 mV induces I_Ca,L, which decreases during stretching (indicated by a negative blue current wave compared to a negative red wave). A.2 – I/V curve of I_ns before (blue triangles) and during (red triangles) stretching, as well as I_Ca,L before (blue circles) and during (red circles) stretching. B: (8 μm stretch). B.1 - The time course of the membrane current before and during stretching, which results in a greater reduction of I_Ca,L compared to A.1. Notations as in A.1. B.2 – I/V curves of I_ns and I_Ca,L before and during stretching. Notations as in A.2. C: (10 μm stretch). C.1 – The time course of the membrane current in control and during stretching. I_Ca,L decreases with increasing stretching. Notations as in A.1. C.2 – I/V curves of I_ns and I_Ca,L before and during stretching. Notations as in A.2