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Fig. 2 | Biology Direct

Fig. 2

From: Long noncoding RNA KCNMA1-AS1 promotes osteogenic differentiation of human bone marrow mesenchymal stem cells by activating the SMAD9 signaling pathway

Fig. 2

KCNMA1-AS1 promotes osteogenic differentiation of hBMSCs in vitro. A- Transfection efficiency of KCNMA1-AS1 overexpression and KCNMA1-AS1 knockdown was measured by qPCR, normalized to GAPDH. B-E- Relative mRNA levels of COL1A1 (B), RUNX2 (C), OSX (D), and OPN (E) in hBMSCs transfected with lentivirus measured by qPCR after one week of osteogenic induction, normalized to GAPDH. F and G- The protein levels of COLA1, RUNX2, OSX, and OPN in hBMSCs transfected with lentivirus detected by western blot after one week of osteogenic induction. GAPDH was used as the internal reference. H and I ALP staining (H) and ALP activity (I) in hBMSCs transfected with lentivirus after one week of osteogenic induction. Scale bar of microscopical images, 100 μm. J and K- ARS staining (J) and ARS quantification (K) in hBMSCs transfected with lentivirus after two weeks of osteogenic induction. Scale bar of microscopical images, 100 μm. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001, in comparison to NC/sh-NC.

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Biology Direct

ISSN: 1745-6150

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