Fig. 1

KCNMA1-AS1 is upregulated when hBMSCs undergo osteogenic differentiation. A- Differentially expressed lncRNAs analyzed by qPCR after 14 days of osteogenic induction. B- Relative expression levels of KCNMA1-AS1 were measured using qPCR, and Glyceraldehyde 3-phosphate dehydrogenase (GAPDH) was used for normalization. C-F- Relative mRNA levels of COL1A1 (C), RUNX2 (D), OSX (E), and OPN (F) measured through qPCR, normalized to GAPDH. G and H- The protein levels of COL1A1, RUNX2, OSX, and OPN were detected by western blot. The internal reference is GAPDH. I and J- ALP staining (I) and ALP activity (J). hBMSCs cultured in osteogenic medium (OM) or growth medium (GM) for a week. Scale bar of microscopic images, 100 μm. K and L- ARS staining (K) and ARS quantification (L). hBMSCs were grown in an osteogenic medium (OM) or growth medium (GM) for two weeks. Scale bar of microscopical images, 100 μm. M-O- Correlation of the expression of KCNMA1-AS1 with that of COL1A1 (M), RUNX2 (N), and OSX (O) during osteogenic differentiation. ns: not significant, *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001, compared with 0 day/GM.