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Fig. 4 | Biology Direct

Fig. 4

From: Overexpression of ZNF488 supports pancreatic cancer cell proliferation and tumorigenesis through inhibition of ferroptosis via regulating SCD1-mediated unsaturated fatty acid metabolism

Fig. 4

ZNF488 upregulation of SCD1 promotes unsaturated fatty acid metabolism. A The abundance of palmitic, palmitoleic, stearic and oleic acid was measured in siCtrl, siZNF488#1 and siZNF488#2 MIAPaCa-2 and PANC-1 cells. B The abundance of palmitic, palmitoleic, stearic and oleic acid measured in Ctrl and ZNF488 overexpressing MIAPaCa-2 and PANC-1 cells. C MDA levels were checked in ZNF488 knockdown and overexpressing MIAPaCa-2 and PANC-1 cells. D, E The mRNA and protein expression of SCD1 was detected in ZNF488 knockdown and overexpressing MIAPaCa-2 and PANC-1 cells. F Luciferase reporter assays were performed to check the regulation of ZNF488 on the transcription activity of SCD1 gene. G ChIP-qPCR assay was performed to detect the interaction of ZNF488 with the promoter sequence of SCD1. *p < 0.05. **p < 0.01

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