Fig. 4
Lipid profiles of COS-7 and U2OS cells differ drastically and are differentially affected by LONP2 silencing. Untargeted lipidomic analysis of n = 5 independent cell experiments for each group under the basal condition of after LONP2-silencing for which the final dataset included 2,094 lipid features (Table S2). a Principal component (PC) score plot. b Log2 (fold changes) of 206 lipid features annotated by MS/MS and data alignment using our in-house database, and grouped by lipid subclass, whereby red and black dots indicate lipids that reach or not our selected significance threshold for the indicated comparison, Pcorr-value < 0.1. c Box plots of unique lipids annotated by MS/MS from the 100 most significant lipid features discriminating LONP2-silenced COS-7 cells (top) and U2OS cells (bottom) from NT-silenced cells. The midline represents the median fold change vs. NT-silenced cells, the box represents the interquartile range between the first and third quartile, and the whiskers represent the lowest or highest values. d Immunoblotting of cholesterol sensor INSIG1, vinculin used as a loading control. Abbreviations: sphingolipid (SP), sterol lipid (ST), glycerolipid (GL), glycerophospholipid (GP), fatty acyls (FA), sphingomyelin (SM), ceramide (Cer), glucosylceramide (GlcCer), cholesterol (Chol), cholesterol ester (CE), triacylglycerol (TG), diacylglycerol (DG), 1-alkyl, 2-acylglycerophosphocholine (PCO-), 1-(1Z-alkenyl), 2-acylglycerophosphocholine (PCP-), 1-alkyl, 2-acylglycerophosphoethanolamine (PEO-), 1-(1Z-alkenyl), 2-acylglycerophosphoethanolamine (PEP-), diacylglycerophosphocholine (PC), diacylglycerophosphoethanolamine (PE), diacylglycerophosphoinositol (PI), monoacylglycerophosphocholine (LPC), monoacylglycerophosphoethanolamine (LPE), diacylglycerophosphoglycerol (PG), free fatty acid (FFA) and acylcarnitine (CAR). All lipid features were categorized into subclasses and labeled based on the LIPID MAPS® Structure Database