Fig. 1

Multipotent differentiation of MSCs isolated from murine thymic tissue and bone marrow. (A, B) Expression of cell surface markers on T-MSCs and BM-MSCs as determined by flow cytometry. (C-E) Morphology was detected after fixation and analyzed with inverted microscope. α-SMA and Nestin expression was determined after fixation and staining with an Alexa Fluor® 488-coupled antibody against α-SMA and Nestin, respectively, nuclei were counterstained with Hoechst 33,342, and then analyzed under fluorescence microscope. Scale bars, 100 μm. (F, G) Adipogenic and osteogenic differentiation of T-MSCs and BM-MSCs was evidenced by Oil Red O-stained fat vacuoles or Alizarin Red S-stained calcium deposits, respectively. Scale bars, 100 μm or 1 mm