From: Biological roles of RNA m7G modification and its implications in cancer
Technique | Quantitative | Sensitivity | RNA type | Resolution | Main principle | References |
---|---|---|---|---|---|---|
Northern blot | yes | low | tRNA | bulk-level RNA | total RNA isolation and RNA blot hybridization | [14] |
Primer extension | no | low | rRNA | fragmented RNA | reverse transcription | [15] |
LC-MS/MS | yes | low | mRNA, tRNA | bulk-level RNA | liquid chromatography combined with mass spectrometry | [6] |
m7G-MERIP-seq | yes | low | mRNA | fragmented RNA | RNA immunoprecipitation | [11] |
m7G-miCLIP-seq | yes | high | mRNA | single-base resolution | immunoprecipitation and individual-nucleotide-resolution cross-linking | [4] |
m7G-MAP-seq | yes | high | tRNA, rRNA | single-base resolution | chemical reduction and reverse transcription | [18] |
m7G-seq | yes | high | mRNA, tRNA | single-base resolution | chemical reduction and reverse transcription | [11] |
AlkAniline-seq | yes | high | tRNA, rRNA | single-base resolution | chemical cleavage | [19] |
BoRed-seq | yes | high | miRNA | single-base resolution | chemical reduction and reverse transcription | [6] |
TRAC-seq | yes | high | tRNA | single-base resolution | chemical cleavage | [17] |
m7G-quant-seq | yes | high | tRNA | single-base resolution | chemical reduction and reverse transcription | [20] |