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Fig. 4 | Biology Direct

Fig. 4

From: Deep learning-assisted high-content screening identifies isoliquiritigenin as an inhibitor of DNA double-strand breaks for preventing doxorubicin-induced cardiotoxicity

Fig. 4

Deep learning-based high-content screening to find protective agents against doxorubicin-induced DNA damage. A Schematic of the high-content screening. B (a) Scatter plot of the primary screening results, where the mean values of the % foci-positive cells analyzed by FociNet are presented as robust percent of samples (RPS), and the blue data points are hit compounds. (b) Cumulative distribution of the primary screening data analyzed by the FociNet. (c) DMSO-treated control wells vs. hit compounds-treated wells. Data are plotted as mean ± SD, n = 36 ~ 54, **P < 0.01 by Mann–Whitney test. (d) Frequency distribution of data from DMSO-treated control wells vs. hit compounds-treated wells. C Seventeen hit compounds from the primary screening. D Effect of kaempferol, kaempferide, and ISL on foci formation induced by 0.5 μM DOX treatment for 1 h in EGFP-53BP1-H9c2, and that on ATP decrease induced by 0.5 μM DOX treatment for 24 h in H9c2. Data are plotted as mean ± SD, n = 3, ##P < 0.01 when compared with the control group; **P < 0.01 when compared with the DOX group

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Biology Direct

ISSN: 1745-6150

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