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Fig. 5 | Biology Direct

Fig. 5

From: Perilipin 5 deficiency aggravates cardiac hypertrophy by stimulating lactate production in leptin-deficient mice

Fig. 5

Inhibiting lactate production ameliorated insulin resistance in Plin5-deficient cardiomyocytes. A The expression levels of AKT and p-AKT in H9C2 cells were analysed by immunoblotting after treatment with various concentrations of lactate in the absence and presence of insulin (n = 3, ***P < 0.001). B, C Wild-type (Plin5+/+) and Plin5-null (Plin5−/−) CMs were cultured in media supplemented with FAss, lactate levels (B) were determined after treatment with 10 μM GSK2837808A, and the expression levels of AKT and p-AKT (C) were detected by immunoblotting (n = 3, *P < 0.05). D–F The NADH levels, NAD + /NADH ratios (D) and lactate levels (E) were determined in wild-type (Plin5+/+) and Plin5-null (Plin5−/−) CMs, and the expression of AKT, p-AKT (F) was detected after treatment with 60 μM etomoxir (n = 3, *P < 0.05, **P < 0.01)

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