Fig. 1

Plin5 deficiency reduced glucose utilization in mouse cardiomyocytes. A Representative images of H&E staining and Oil Red O staining of myocardial tissue from wild-type (Plin5^+/+) and Plin5-knockout (Plin5^−/−) mice. Scale bar, 50 μm. B Quantification analysis of Oil red O staining (n = 3, **P < 0.01). C Triglyceride (TG) levels in myocardial tissue of Plin5^+/+ and Plin5.^−/− mice (n = 6, **P = 0.01). D, E BODIPY 493/503 dye staining in neonatal mouse cardiomyocytes after treatement with FAs for 24 h and quantification analysis. Scale bar, 10 μm. F, G Fluorescence photomicrograph (F) and intensity analysis (G) of neonatal mouse cardiomyocytes after 2-NBDG administration for 30 min. Scale bar, 50 μm. (n = 6 in G, **P < 0.01). H Neonatal mouse cardiomyocytes were cultured in DMEM with 25 μm glucose and treated with FA for the indicated times, and the glucose concentration in the medium was determined (n = 3, *P < 0.05). I, J Fluorescence photomicrograph (I) and intensity analysis (J) of the control (EV) and Plin5-overexpressing (Plin5) H9C2 cells after 2-NBDG administration. Scale bar, 50 μm. (n = 6 in J, **P < 0.01). K Glucose concentration in the medium of Plin5-overexpressing H9C2 cells treated with FAs (n = 3, *P < 0.05)