Fig. 5

Cx43 hemichannel activation leads to subcellular re-distribution of caveolin-1 (Cav-1) and Cx43 in migrating endothelial cells. a, Immunofluorescence analysis of the subcellular distribution of Cav-1 (red) and Cx43 (green) in control conditions and after the treatment with 300 µM TAT-Gap19 (Gap19), a specific blocker of Cx43 hemichannels. Cell nuclei are highlighted by the staining with DAPI (blue). The square indicatres the area that was enlarged on the right. b and c, Densitometric analysis of Cx43 (b) and Cav-1 (c) signal in the rear and anterior part of endothelial cells of the migration front in control conditions and in the presence of Gap19. Numbers inside the bars indicate the n value. Values are means ± SEM. **, P < 0.01 vs. Anterior by paired Student’s t-test