Fig. 2

Cx43 hemichannels are not involved in endothelial cell proliferation. a, Analysis of endothelial cell proliferation by the bromodeoxyuridine (BrdU) incorporation assay in control conditions and after the treatment with the Cx43 hemichannel blocking peptide TAT-Gap19 (Gap19, 300 µM). Cell proliferation was evaluated in primary cultures of mesenteric endothelial cells at 40% or at 80% of confluence. b, Representative images of the immunofluorescence detection of BrdU incorporation into endothelial cells of the migration front and the monolayer in control conditions or in the presence of Gap19. c, Fluorescence intensity analysis of the experiments shown in b. BrdU+ denotes endothelial cells positive for BrdU. Numbers inside the bars indicate the n value. Values are means ± SEM