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Fig. 6 | Biology Direct

Fig. 6

From: Analysis of state 1—state 2 transitions by genome editing and complementation reveals a quenching component independent from the formation of PSI-LHCI-LHCII supercomplex in Arabidopsis thaliana

Fig. 6

PAM fluorescence traces recorded during state 1—state 2 transition measurement protocol. The PSII fluorescence quenching kinetics upon state 1–2 transition were derived from the PAM recording used to estimate the qT, 1-qP and 1-qL parameters (Fig. 5). The superimposition of far-red (FR) light to a basal actinic light source (AL) was employed to induce the state 1, while removal of the former induced a state 2 condition. All genotypes with reduced qT values (Fig. 5) also exhibited altered fluorescence trace profiles. The kostn7 mutant displayed the most extreme fluorescence phenotype, while the koLhcb2 and cB2.1T40V both displayed a sustained fluorescence trace profile during state 1—state 2 transition, consistent with their inability to assemble the PSI-LHCI-LHCII supercomplex, reduce the PSII antenna absorption cross section and relieve the over-reduction of the plastoquinone pool

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