Fig. 3

ILC1s promote M1-like phenotype macrophage polarizing in vitro. Sorted ILC1s (1 × 10⁵) from mouse spleen co-cultured with macrophage cell line RAW264.7 (2 × 10⁴) and bone marrow-derived macrophage (BMDM) for 24 h. A representative flow cytometry graphs and statistical chart of the expression of CD206 and CD86 in ILC1s co-cultured RAW264.7 and control RAW264.7. B representative flow cytometry graphs and statistical chart of the expression of CD206 and CD86 in ILC1s co-cultured BMDM and control BMDM. C, D 10 µg/ml anti-IFN-γ and TNF-α were added to co-culture system, flow cytometry was used to detected the expression of CD206 and CD86 in RAW264.7 and BMDM of different treated groups. E, F Western blotting was used to detect the expression of iNOS and Arg-1 in ILC1s co-cultured, anti-IFN-γ and TNF-α treated co-culture system, and control RAW264.7. G, H western blotting was used to detect the expression of iNOS and Arg-1 in ILC1s co-cultured, anti-IFN-γ and TNF-α treated co-culture system, and control BMDM. All the experiments repeated for 3 times