Fig. 5

iPSC-derived cardiac fibroblasts from DMD patients showed a reduced number of mitochondria. A: Western blot (left) and quantification(right) of HSP60 and VDAC1 protein expression in iPSC-derived cardiac fibroblasts. Quantification was performed on six biological samples obtained from multiple differentiation batches. Non parametric t-test was applied to determine the statistically significant differences between DMD and control. *p < 0.05, **p < 0.01. B: The relative abundance of mtDNA genes COX1 and ND1 using nuclear gene β globulin as a reference, was measured by PCR in hiPSC-fibroblasts derived from control and DMD patients. Non parametric t-test was applied to determine the statistically significant differences between DMD and control. *p < 0.05. Western blot quantification of MFN2 (C) and DRP1 (D) protein expression in iPSC-derived cardiac fibroblasts. Non parametric t-test was applied to determine the statistically significant differences between DMD and control