Fig. 3

Hsa_circ_0067842 can regulate the immune escape of BC. MDA-MB-468 or MCF-7 cells in different treatment groups were co-cultured with isolated PBMCs. A Ki-67 staining was used to observe the PBMCs proliferation. B Flow cytometry was used to determine the percentage of CD8⁺ T cells in PBMCs. C LDH cytotoxicity assay was used to detect the cytotoxicity of PBMCs. Quantitative data were presented as the mean ± SD, ^*p < 0.05, ^**p < 0.01, ^***p < 0.001 and ^****p < 0.0001