Fig. 2
Mineralization microenvironment’s influence on tooth germ isolated at E14.5 and P7. A Tooth germs of first molars isolated from E14.5 mandibles were incubated with (200 ng/ml, b1) or without (0 ng/ml, a1) BMP2 for 7 days. HE staining of tissue sections demonstrated structure disruption with loss of polarity and continuity after stimulation with 200 ng/ml of BMP2 (b2) compared with control (a2). Masson’s trichrome staining high-lighted the disruption to the dentin structure in 200 ng/ml BMP2 treated group (b3) compared with control group (a3). B RT-qPCR comparing tooth development related markers, including Dspp(a), Dmp1(b), Ambn(c), and osteogenic markers including Osx(d), Alp(e), and Runx2 (f) between 200 ng/ml BMP2 treated E14.5 tooth germ and control group. C Tooth germs of first molars isolated from P7 mandibles were incubated with (200 ng/ml, b1) or without (0 ng/ml, a1) BMP2 for 7 days. HE staining and Masson's trichrome staining indicated increased dentin formation in 200 ng/ml BMP2 treated group (b2 and b3) compared with control group (a2 and a3). D RT-qPCR comparing tooth development related markers and osteogenic markers including between 200 ng/ml BMP2 treated P7 tooth germ and control group. Dspp, dentin sialophosphoprotein; Dmp1, dentin matrix acidic phosphoprotein 1; Ambn, ameloblastin; Osx, osterix; Alp, alkaline phosphatase; Runx2, Runt-related transcription factor 2. n = 5, *p < 0.05, **p < 0.01, ***p < 0.001. ns no significance. Scale bars: 200 μm (A, C)