Fig. 7

Increased miR-146a-5p in microglial exosomes inhibit neuroinflammation in mice exposed to IH8w. (a) Schematic diagram of experimental design. (b) Immunofluorescence staining showed that the co-expression PKH26 and NeuN, indicating that the exogenous exosomes were absorbed by neurons of mice treated with IH8w. (c) The expression levels of miR-146a-5p in the brain of control, IH8w, IH8w + Exosome, IH8w + Exo-miR-146a and IH8w + MCC950 mice were detected by RT-PCR. (d-e) The mRNA expression levels of NLRP3, IL-18, IL-1β, TNFα in the brain of different groups of mice were determined by qRT-PCR. (f-g) The protein expression of NLRP3, cleaved caspsase1 in the brain of five groups of mice were performed by western blotting. Data are presented as mean ± SD (n = 4–8) and analyzed using one-way ANOVA with Tukey’s post hoc test or Dunnett’s T3 post hoc test. *P < 0.05, **P < 0.01, and ***P < 0.001