Fig. 1

NLRP3 inflammasome and neuroinflammation were detected in mice exposed to IH8w. (a) Schematic diagram of experimental design, and the mouse IH cycling protocol used in this study. (b) Western blot was used to detect the levels of NLRP3 inflammasome proteins (NLRP3, Cleaved caspase1, GSDMD, ASC) at different time points after IH. (c) Protein levels were normalized to β-actin, and the results showed as fold change of control. (d-e) The time profiling of IL-1β, IL-18 mRNA levels in the brain of mice exposed to IH were performed by qRT-PCR. (f) Representative immunofluorescence staining images of NLRP3 co-localized with neurons in the dentate gyrus (DG) region of the hippocampus of mice exposed to CIH or normoxia for 8 weeks. Scale bar: 50 μm. Data are presented as mean ± SD (n = 4–8) and analyzed using one-way ANOVA with Tukey’s post hoc test. *P < 0.05, **P < 0.01, and ***P < 0.001