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Fig. 5 | Biology Direct

Fig. 5

From: ANKRD29, as a new prognostic and immunological biomarker of non–small cell lung cancer, inhibits cell growth and migration by regulating MAPK signaling pathway

Fig. 5

Overexpression of ANKRD29 repressed NSCLC cell migration. A Representative photograph demonstrating the morphological change of H1975 after ANKRD29 overexpression in bright field. B-C SPC-A1 and H1975 cells’ migration abilities were inhibited after the overexpression of ANKRD29 by wound-healing assays. (C) Quantification data for (B). D-E ANKRD29 Overexpression suppressed SPC-A1 and H1975 cells’ migration abilities by trans-well assays. F The expressions level of EMT-related protein Slug and Snail were determined by Western blotting. G IC50 values were determined by SRB assays in ANKRD29 overexpressing cell and control group of SPC-A1. Scale bar = 50 μm. Bars are the mean value ± SD. ns = no significant. * P < 0.05, ** P < 0.01, *** P < 0.001

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