Fig. 1

XIST upregulated in HLF tissues and associated with the LF hypertrophy and fibrosis. A–C The volcano plots visualized the expression of lncRNAs, miRNAs and mRNAs between 3 HLF tissues and 3 control tissues. The red and blue dots in the volcano plots indicated statistically significant upregulated and downregulated RNAs, respectively. D Construction of hub mRNAs-miRNAs-lncRNAs competing endogenous RNA (ceRNA) networks in HLF. Ellipses, quadrangle, and triangles represented dysregulated lncRNAs, dysregulated miRNAs, and dysregulated hub mRNAs, respectively. Pink and blue represented upregulated and downregulated RNAs, respectively. E RT-qPCR was used to verify the expression of dysregulated lncRNAs from the ceRNA network in 3 HLF tissues and 3 control tissues. F The XIST expression in 25 HLF tissues and 16 control tissues was detected by RT-qPCR, and found that XIST was upregulated in HLF tissues. G Pearson correlation coefficient was used to analyze the correlation between the XIST expression and LF hypertrophy or LF fibrosis. H RT-qPCR was used to measure the XIST expression in normal LF tissue-derived cells (non-HLF cells) and HLF tissues-derived cells (HLF cells), and found that XIST expression levels was markedly upregulated in HLF cells compared to non-HLF cells. ***P < 0.001