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Fig. 3 | Biology Direct

Fig. 3

From: LncRNA5251 inhibits spermatogenesis via modification of cell-cell junctions

Fig. 3

Overexpression of lncRNA5251 disrupted spermatogenesis in F0 mice. (a) The protein staining of DDX4, PGK2 and SYCP3 in F0 mice testis after shRNA treatment detected by IHF. (b) The quantitative data for IHF in (a). (c) Gene ontology (GO) enrichment of up-regulated genes after knockdown of lncRNA5251. (d) GO enrichment of decreased genes after overexpression of lncRNA5251. (e) The protein levels of CADM2, CX43, CYP11A1 and BCL-XL detected by WB in F0 mouse testes. (f) The quantitative data for WB in (e). Data present as Average ± SEM. a, b indicate a significant difference among different treatments (p < 0.05)

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