Fig. 5

Detection of SAM and SAH by UPLC-QTOF-MS. A Schematic representation of the methionine cycle, the proposed inhibitory action of AHCYL1 on AHCY, and the interaction with SAH. MTR: Methionine synthase. MAT2A: Methionine adenosyl transferase 2A. Normalized chromatographic peak areas for SAM (B) and SAH (C) for NT and KD-AHCYL1-4 cells (n = 16 for each cell line, p = 0.026). D SAM/SAH ratio for NT and KD-AHCYL1-4 cells calculated for each sample (n = 16, p = 0.007). *p ≤ 0.05, **p ≤ 0.01. E Western blot analysis of AHCYL1 protein level and H3K4,-K9 and -27 methylation levels. Total histone H3 was used as a loading control. The blot corresponds to a representative experiment of two or three blots with similar results