Fig. 2

SNHG17 transfers from cytoplasm to nucleus post radiation. a Representative photographs of the location of SNHG17 in cells prior and post radiation through Fluorescence in situ hybridization (FISH) assay by confocal microscopy. b Quantitative analysis of SNHG17 expression in cytoplasm and nucleus prior and post radiation through nucleocytoplasmic separation assay. c Representative photographs of migration ability after knockdown SNHG17 expression in A549 cells with or without 6 Gy treatment through scratch wound healing migration assay. d Representative photographs of cell apoptosis at 24 h after knockdown SNHG17 expression in A549 cells with or without 6 Gy treatment. e Representative photographs of cell cycle after knockdown SNHG17 expression in A549 cells with or without 6 Gy treatment through flow cytometric cell cycle assay. GAPDH served as the cytoplasmic expression control and U6 severed as the nuclear expression control. Error bars represent the SEM of the mean of 3 independent experiments. Data are means ± SD (standard deviation). N = 3 independent experiments, Student’s two-tailed unpaired t test was used to compare differences between two groups. *p < 0.05