Fig. 5

Rab32 Promotes Autophagic Degradation of MERC membranes. A Representative images from electron microscopy of stably expressing control pIRES2 or Rab32Q85L cells. Boxes indicate ER-mitochondria contact sites ≤ 50 nm apart. Top: quantification of length of mitochondrial membrane 10-50 nm apart from ER (see below left) divided by average distance of ≤ 50 nm between the two organelle membranes (see below right). n = 100; ***p ≤ 0.0001. Insets show zoomed areas from boxed areas, highlighting typical ER structures in the proximity of mitochondria. B Immunoblot of transfected wild type and knockout PACS-2 HeLa and mitofusin-2 mouse embryonic fibroblast (MEF) cells. p62 was probed for comparison and to track Rab32Q85L-induced autophagy