Fig. 4
CB2 high expression represented a better prognosis of HCC patients and CB2 knockdown reversed the inhibition of HCC cell proliferation induced by MDA19. a Kaplan-Meier plotter (http://kmplot.com/analysis/) is a bioinformatics website that assesses the effect of 54,675 genes on survival using 10,461 cancer samples. The survival curves of HCC patients with high or low CB2 mRNA level were acquired by searching on Kaplan-Meier plotter; (Affy id/Gene symbol: 1269_s_at; Survival: RFS (n = 364); Follow up threshold: all; and Auto select best cutoff and user selected probe set were selected to generate these figures); (b) The correlation of CB2 expression with clinical factors of HCC patients; (c) Three siRNAs targeting CB2 were synthesized and introduced into Hep3B and HepG2 cells. The mRNA expression of CB2 was detected by qRT-PCR. d CB2 was knocked down by using RNAi technology and western blot was used to detect the interference efficiency on protein level; (e) CCK8 was used to detect cell proliferation of HCC cells in NC (negative control), CB2-KD and MDA19 + CB2-KD groups. NC: HCC cells were transfected with siNC (50nM); CB2-KD: HCC cells were transfected with CB2 siRNA (50nM); MDA19 + CB2-KD: HCC cells were transfected with CB2 siRNA (50nM) and treated with MDA19 (30μM for Hep3B and 40μM for HepG2). All experiments were performed at 3 times. *P < 0.05