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Table 3 Divergence analysis for GGT subfamilies: Divergence type I and type II analyses of two clusters comparisons are shown here in details

From: “Phylogenetic and evolutionary analysis of functional divergence among Gamma glutamyl transpeptidase (GGT) subfamilies”

Bacteria 1/Bacteria 4-Extremophile

Bacteria 1/Eukaryote

FD I

FD II

FD I

FD II

θ I = 0.49 ± 0.08

θ II =0.31 ± 0.09

θ I = 0.58 ± 0.12

θ II = 0.31 ± 0.08

P = 0.75

R = 9.50

P = 0.70

R = 10.35

Site

E c

B h

Site

E c

B h

Site

E c

Hm

Site

E c

Hm

G 1

G 1

G 1

G 1

G 1

G 1

G 1

G 1

G 1

G 1

G 1

G 1

415

P291

P268

215

R114

S88

544

V396

V386

540

T392

A382

463

A335

V309

540

T392

V359

546

D398

A388

631

S481

A471

465

R337

G311

541

H393

Y360

629

T479

V367

638

I488

T478

474

F346

D320

555

T407

I374

632

P482

A472

 

478

D350

S319

559

N411

Y378

641

V491

T481

 

535

E387

P354

611

S462

I422

725

I556

I547

 

576

N428

Q395

631

S481

V438

758

E563

G553

 

583

S435

S402

632

P482

M439

 

602

A453

A410

637

I487

Q444

 
 

638

I488

P445

 

G 2

G 2

G 2

G 2

G 2

G 2

G 2

G 2

G 2

G 2

G 2

G 2

264

Y161

Y133

184

A84

T59

163

I65

A57

184

A84

G76

275

V170

L142

187

H87

T64

287

I182

P169

187

H87

N79

276

Q171

A143

190

A90

N66

342

L231

Y217

188

P88

A80

287

I182

P154

196

G95

D71

383

A269

N255

190

A90

S82

349

G238

N215

213

D112

N86

409

Y285

V272

191

G91

M83

351

D240

E217

216

E115

G89

491

K361

A352

197

G97

L89

398

R283

R260

338

L227

A205

647

N497

Y487

213

D112

N105

409

Y285

Y262

389

R275

V252

687

D532

A522

249

G147

A133

432

E308

K284

543

S395

A362

719

G551

A542

261

L158

H145

440

G316

D292

579

M431

G398

 

264

Y161

H148

486

N356

S330

641

S465

H419

 

266

T163

R150

487

K357

D331

665

H515

Q472

 

278

A173

S160

489

Y359

Y333

 

316

K206

C192

545

V397

A364

 

362

A248

T234

550

N402

N369

 

389

R275

I261

624

K474

Q431

 

395

G280

I267

649

I499

I456

 

398

R283

G270

675

D521

K477

 

408

G284

D271

687

D532

D488

 

414

M290

P277

759

L564

L524

 

418

P292

A279

 

416

S294

L281

 

423

H299

V285

 

432

E308

K294

 

464

D336

K326

 

487

K357

E348

 

585

K437

P427

 

608

R459

Q448

 

643

Q493

L483

 

650

D500

W490

 

675

D521

N511

 

761

G566

A556

  1. θI and θII >0 are observed for type I and type II analysis respectively in each case. Corresponding residues are marked on the two representative proteins of the compared clusters with respect to predicted divergence sites by FDI and FDII methods. Divergence sites predicted through the analysis are divided into two groups, G1 and G2, where G1 represents the sites that are close to the known functional residues forming the substrate binding cavity and G2 represents all other sites.  Ec: gamma glutamyl transpeptidase of Escherichia coli. Bh: Gamma glutamyl transpeptidase of Bacillus halodurans. Hm: gamma glutamyl transpeptidase of human