Table 2 Comparison of the sequence alignment between the Drosophila simulans and Wolbachia cox1 genes in the six possible reading frames
Nucleotidic position on the Drosophila cox1 gene | 1-381 | 382-684 | 685-988 | 989-1290 | 1291-1536 |
Number of nucleotides by region | 381 | 303 | 303 | 303 | 246 |
% of identical nucleotide in the alignment | 60.9 | 66.7 | 62.7 | 57.4 | 50.8 |
Gap/indel | 0 | 0 | 3 | 0 | 0 |
+1 reading frame (cox1) | Â | Â | Â | Â | Â |
a.a. position on the Drosophila Cox1 protein | 1-127 | 128-228 | 229-329 | 330-430 | 431-512 |
Number of a.a. by region | 127 | 101 | 101 | 101 | 82 |
% of identical a.a. | 60.6 | 68.3 | 56.4 | 46.5 | 36.6 |
% of identical and functionally similar a.a. | 81.9 | 91.1 | 94.0 | 73.3 | 61.0 |
Gap/indel | 0 | 0 | 1 | 0 | 0 |
+ 2 reading frame | Â | Â | Â | Â | Â |
% of identical a.a. | 23.6 | 27.7 | 34.6 | 27.7 | 16.0 |
% of identical and functionally similar a.a. | 36.2 | 38.6 | 48.5 | 41.0 | 38.3 |
Gap/indel | 0 | 0 | 5 | 3 | 0 |
% of stop codon in Wolbachia sequence | 11.0 | 5.9 | 7.9 | 5.9 | 3.7 |
% of stop codon in Drosophila sequence | 15.0 | 8.9 | 9.9 | 14.8 | 7.4 |
+ 3 reading frame | Â | Â | Â | Â | Â |
% of identical a.a. | 25.4 | 22.7 | 28.7 | 17.8 | 18.3 |
% of identical and functionally similar a.a. | 46.8 | 38.6 | 44.6 | 38.6 | 43.9 |
Gap/indel | 0 | 0 | 1 | 0 | 1 |
% of stop codon in Wolbachia | 7.1 | 4.9 | 3.9 | 7.9 | 6.0 |
% of stop codon in Drosophila | 2.4 | 1.0 | 2.0 | 3.0 | 1.2 |
-1 reading frame | Â | Â | Â | Â | Â |
% of identical a.a. | 26.2 | 24.7 | 30.7 | 21.8 | 19.3 |
% of identical and functionally similar a.a. | 51.2 | 48.5 | 49.5 | 49.5 | 37.8 |
Gap/indel | 1 | 0 | 1 | 0 | 0 |
% of stop codon in Wolbachia sequence | 8.7 | 6.9 | 3.0 | 6.9 | 4.9 |
% of stop codon in Drosophila sequence | 11.1 | 13.9 | 9.9 | 12.9 | 11.0 |
-2 reading frame (= gau ORF) | Â | Â | Â | Â | Â |
% of identical a.a. | 42.8 | 47.5 | 41.6 | 34.6 | 28.0 |
% of identical and functionally similar a.a. | 58.7 | 57.4 | 48.5 | 39.6 | 48.8 |
Gap/indel | 0 | 0 | 1 | 0 | 0 |
% of stop codon in Wolbachia sequence | 3.2 | 1.0 | 7.9 | 8.9 | 3.7 |
% of stop codon in Drosophila sequence | 1.6 | 1.0 | 3.0 | 4.0 | 3.7 |
-3 reading frame | Â | Â | Â | Â | Â |
% of identical a.a. | 23.1 | 26.7 | 25.7 | 19.8 | 17.3 |
% of identical and functionally similar a.a. | 38.6 | 35.6 | 43.6 | 39.6 | 33.3 |
Gap/indel | 0 | 10 | 1 | 0 | 1 |
% of stop codon in Wolbachia sequence | 10.2 | 10.9 | 10.9 | 8.9 | 4.9 |
% of stop codon in Drosophila sequence | 6.3 | 9.9 | 10.9 | 10.9 | 7.4 |