Figure 6

Column-versus row-analysis of TFAM's output. The y axis indicates the percentage of non-cognate amino acids with greater amino acylation potential than the cognate according to TFAM, assuming competition among tRNAs for tRNA synthetases (column-analysis of output in Table 2, see text). The x-axis is the percentage of non-cognate amino acids with greater aminoacylation potential than the cognate supposing competition among tRNA synthetases for tRNAs (row-analysis of Table 2). This graph shows that for many sense tRNAs for which classical interpretations of TFAM's output (row analysis of Table 2) yield a poor prediction of the cognate, assuming that tRNAs compete for tRNA synthetases explains apparent high misacylation rates.