Locality versus globality in bacterial signalling: can local communication stabilize bacterial communities?
© Venturi et al. 2010
Received: 29 March 2010
Accepted: 27 April 2010
Published: 27 April 2010
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© Venturi et al. 2010
Received: 29 March 2010
Accepted: 27 April 2010
Published: 27 April 2010
Microbial consortia are a major form of life; however their stability conditions are poorly understood and are often explained in terms of species-specific defence mechanisms (secretion of extracellular matrix, antimicrobial compounds, siderophores, etc.). Here we propose a hypothesis that the primarily local nature of intercellular signalling can be a general mechanism underlying the stability of many forms of microbial communities.
We propose that a large microbial community can be pictured as a theatre of spontaneously emerging, partially overlapping, locally recruited microcommunities whose members interact primarily among themselves, via secreted (signalling) molecules or cell-cell contacts. We hypothesize that stability in an open environment relies on a predominantly local steady state of intercellular communication which ensures that i) deleterious mutants or strains can be excluded by a localized collapse, while ii) microcommunities harbouring useful traits can persist and/or spread even in the absence of specific protection mechanisms.
Some elements of this model can be tested experimentally by analyzing the behaviour of synthetic consortia composed of strains having well-defined communication systems and devoid of specific defence mechanisms. Supporting evidence can be obtained by in silico simulations.
The hypothesis provides a framework for a systematic comparison of bacterial community behavior in open and closed environments. The model predicts that local signalling may enable multispecies communities to colonize open, structured environments. On the other hand, a confined niche or a host may be more likely to be colonized by a bacterial mono-species community, and local communication here provides a control against spontaneously arising cheaters, provided that survival depends on cooperation.
This article was reviewed by G. Jékely, L. Aravind and E. Szathmáry (nominated by F. Eisenhaber)
Many bacteria and other unicellular organisms live in large, multispecies communities in which the participants jointly exploit the resources. Multispecies consortia are a major form of bacterial life and often contain hundreds of different species that share secreted materials in a densely packed environment. Social behaviour must be an essential trait throughout bacterial evolution , however there is no sufficient experimental evidence to explain why such consortia can be stable against environmental challenges or against the emergence of non-cooperating cheater mutants. Current explanations suggest that specific, species-dependent defence mechanisms or simple mechanical protection present in particular communities (such as seen in biofilms) may be the major factors underlying the stability [2–4]. One of our motivations is to look for alternative, more general explanations.
Many prokaryotes possess inter-cellular signalling systems which allows species to colonise new habitats, to invade hosts and to spread over surfaces [5–7] A typical example is quorum sensing (QS) which enables bacteria to switch from low activity to high activity regimes using signalling molecules as well as various public goods (e.g. surfactants, enzymes, siderophores) that facilitate movement, nutrient uptake amongst other things [7, 8]. Signalling molecules are believed in most cases to be transferred by diffusion in the medium surrounding the bacterial populations so their local concentration can vary according to local cell density, positional and/or spatial constraints (for a recent review see ). It is also known that a large part of sequenced bacterial species contain sensors for exogenous signals produced by other bacteria or potential host organisms [10, 11]. In line with our reasoning is that intercellular signalling may be in part responsible for the stability of bacterial consortia.
Recently we presented an agent based model of bacterial quorum sensing  in which the bacterial agents randomly moving on semi-solid agar surfaces, in a process called swarming ; in this scenario, bacteria are communicating via diffusible molecules (in this case the signals were N-acyl-homoserine lactones). In this model, food is converted into diffusible molecules, cellular motion as well as stored energy that ultimately fuels division of the cells. According to this model, swarming bacterial models were seen to spontaneously form an "active zone", a well-defined region of the surface, in which both diffusible molecules and bacterial cells were present in sufficient quantities so as to maintain the community in a quasi steady state. In this state, cell division must compensate or exceed the death rate and production of chemical signals and public goods must compensate for the losses caused by diffusion and decay. This situation is analogous to (quasi) steady states observed in many physical, chemical and social systems[14, 15]. It is worth noting that our model is one in a long series of agent-based models in which intercellular communication is primarily local, i.e. it is directed to a finite neighbourhood around each agent [16–20]. In line with the above, we recently demonstrated that collapse of a swarming community can localize cheater mutants . This leads us to the hypothesis that local communication, hence local community formation may be one of the driving forces responsible for the stability of bacterial consortia. More specifically we suggest that local communication gives rise to a quasi steady state of cooperative exchanges, and the kinetics of cooperation provides protection to bacterial consortia.
We therefore propose that local bacterial communication within large consortia ensures that microcommunities are recruited and eliminated locally and this phenomenon conveys a measure of kinetic stability to microbial consortia even in the absence of specific mechanisms (antibiotics, extracellular matrices etc.).
Some elements of this model can be tested experimentally by comparing the behaviour of synthetic consortia living in open and closed environments. We suggest that construction of strains having well-defined communication systems and devoid of specific defence mechanisms can provide suitable model systems to test the hypothesis on smaller consortia containing a few species. Swarming communities of bacteria, such as Pseudomonas aeruginosa , Serratia sp , Proteus mirabilis  etc. appear to be especially useful models since their signalling systems are well understood [26–28]. The behaviour of large, multispecies communities may be a challenge since some of the participating species will inevitably contain specific mechanisms that may complicate the interpretation of the results. Supportive evidence can be collected for instance via the study of mutation rates: communities dependent on cooperation in an open environment should have a smaller number of mutants as compared to the same community in a well-mixed (i.e. globally communicating) environment. Another experiment might include the study of mutants varying in terms of fitness. Our hypothesis predicts that greedier, faster dividing mutants that do not contribute to the production of public goods are more likely to collapse a community than mutants that divide only slightly faster than the wild type cells.
Explaining the stability and resilience of biological communities has been a long-standing aspiration from both theoretical and clinical perspectives. Our hypothesis is meant to point out that stability of microbial communities can be formulated in terms of an exchange of signals and secreted molecules, and the local nature of the communication provides a measure of stability to the community. In contrast to specific defense mechanisms, the present hypothesis is not concerned with the species-specific properties, thus it can be applied also to multispecies consortia. We think that the general mechanism outlined here complements rather than replaces other known, specific mechanisms.
The present hypothesis refers to an exchange of molecules and/or signals between bacteria which is an ad hoc short term event. In this sense, we consider a community stable at the level of functional repertoire if it collectively possesses the traits necessary to survive in a given environment. On the other hand, a community can be also considered stable at the metagenomic level if its members collectively contain a certain set of genes sufficient to ensure the survival of the community. However, the hypothesis is not meant to explain the evolutionary fate of the underlying mechanisms and/or the evolution of cooperation (an excellent overview is in ).
The hypothesis provides a framework in which the stability of communicating cellular communities (bacterial consortia, host-pathogen ensembles etc.) can be approached and new clinical intervention strategies can be formulated. For instance, QS has been implied as the mechanism by which bacteria establish themselves in their hosts, and QS mutants have been suggested as suitable tools of intervention that can interfere with the infectivity of pathogenic bacteria by competing out wild type pathogens within the host . On the other hand, our hypothesis suggests that QS mutants may not be necessarily able to overtake an entire habitat, if cooperation is necessary for colonizing the host organism.
Our hypothesis does not imply that microbial communication is exclusively local, we rather suggest that local interactions dominate some of the essential features of the community. We simply imagine bacterial communities to have a balance of local and global communication in which interactions within distant cells are not as frequent as those within immediate neighbours. This can be pictured as a colony being composed of many overlapping communities defined by their communication zone of intercellular signalling. As this radius increases, communication becomes increasingly global. For instance, contact-based signalling between cells  is more local than communication via diffusible signals. If communication breaks down between parts of the community, the group will split into distinct parts, such as may be the case when a part of the community collapses, or when a microcommunity breaks away from the rest of the colony. Also in the predominantly local interactions, there is a measure of globality in as much as the signal can be passed from neighbour to neighbour.
The hypothesis provides a framework for systematic comparison of bacterial community behavior in open and closed environments. The model predicts that local signalling may enable multispecies communities to colonize open, structured environments by putting a security control in place against non-cooperators/cheaters. On the other hand, a relatively closed/confined niche or a host may be more likely to be colonized by a bacterial mono-species community, and local communication here provides a control against spontaneously arising cheaters.
It was pointed out by one of the reviewers of this paper that the local communication/local interactions hypothesis does not rely on community properties that are exclusively bacterium-specific (E. Szathmáry, personal communication). This makes us speculate that a similar reasoning may be applicable to other systems that can be modelled as ensembles of locally interacting entities. Protein structures, protein assemblies can all be pictured as networks dominated or at least influenced by local interactions, and recruitment of molecular assemblies or the overlapping module-structure of protein interaction networks provide further intriguing analogies. Nevertheless, the interpretation of locality and globality may greatly vary from system to system, so such wide analogies need to be interpreted with care.
What is then the difference between the present hypothesis and the current views on biological communities? Current paradigms of biological communities use concepts such as altruism, mutuality, kinship, policing, spite, etc., i.e. phenomenological notions that stress the analogies with social sciences and game theory. In contrast, the key concepts of the local communication hypothesis are diffusion, steady state and signalling, i.e. terms related to an underlying mechanism, borrowed from physicochemistry and information technology. We think the two views complement rather than exclude each other.
Reviewer's comment: In recent years there is mounting evidence that the behavior and biochemistry of bacterial communities have key lessons for understanding diverse evolutionary processes. A key behavioral problem is how diverse bacterial communities show resilience in face of cheaters that maximize their own fitness at the cost of other members of the community. Biochemical evidence, supported by genomics, suggests that several measures such as lineage-specific diversification of siderophores (e.g. the pyoverdine operon in Pseudomonas), and use of mechanical protection as in biofilms might be defense mechanisms of communities. However, it is not clear if these specific defenses are the only explanations for the problem under consideration. The current article proposes a different kind of hypothesis invoking more a general process to explain resistance to destructive behaviors. The current hypothesis is certainly amenable to different kinds of tests and qualifies as a valid proposal in this regard. A more difficult question is how the proposed process interacts with the other more specific explanations. This includes questions such as: Do the specific and general mechanisms act in the same conditions or are they approaches specific to certain environmental conditions? For example, it is possible that the general mechanism dominates in solid substrates such as soil whereas the specific mechanisms are more valid in bulk aqueous media. Again, a specific mechanism such as the formation of an extracellular matrix could potentially be a facet of the more general mechanism proposed in this article.
Authors' response: We speculate that the mechanisms of local collapse/local communication play a role in heterogeneous, open systems and that they cannot play a role in perfectly mixed, homogenous media. In the latter conditions only the specific mechanisms are likely to play a role. We considered extracellular matrix formation as a specific defense mechanism, however it is true that it also constrains the diffusion of secreted materials. These points are now addressed in the text.
Reviewer's comment: An optional suggestion to the authors: A schematic figure summarizing the hypothesis might be helpful for the reader to get a quick over view.
Authors' response: We have now included two explanatory figures.
Reviewer's comment: In this paper Venturi et al present a hypothesis that the primarily local nature of intercellular communication in microbioal communities is a source of stability. This is an interesting idea and the hypothesis could stimulate experimental work to test its predictions.
The authors start to present their hypothesis by stating that "We propose that intercellular communication in bacterial consortia is primarily local". I think that the local nature of intercellular communication should not be presented as a part of their hypothesis, but as a known fact arising from physical constraints (diffusion constants etc.). To make this point first, the authors should briefly discuss what is known about the diffusion of various molecules in bacterial communities. A relevant paper about the diffusion of N-acyl-homoserine lactones in biofilms for example is Alberghini et al. FEMS Microbiol Lett. 2009 Mar;292(2):149-61 .
Authors' response: We fully agree that the local nature of AHL diffusion is not part of the hypothesis. We have moved this point to the Background section along with the suggested discussion on diffusion.
Reviewer's comment: In the Background section the author write: "Multispecies consortia often contain hundreds of different species that share secreted materials in a densely packed environment." It would be of course over-ambitious to explain this phenomenon with cooperation and a single hypothesis. Other factors certainly also contribute to the formation of such multispecies consortia. One can for example imagine a mutualistic, syntrophic relationship among several species, where some consume the metabolic waste products of others. These kinds of associations are not endangered by cheaters. Could these maintain the consortia? Maybe. What these interactions cannot explain is the secreted "public goods". This is a cooperative strategy that can be exploited by cheaters. But it is possible that only a few species cooperate, and the others live on them somehow (without necessarily exploiting them). These possibilities, and what the hypothesis tries to explain, could be introduced more carefully.
Authors' response: It was not our intention to imply that local communication is the only mechanism that can contribute to the stability of a community. This is now pointed out in the text. On the other hand, we think it is possible that other forms of coexistence and mutualism can be abused by non-cooperators, and if the coexistence is mediated by an exchange of diffusible materials, the stabilizing effect will be similar to what we suggest for QS bacteria.
Reviewer's comment: The part on the experimental validation of the hypothesis is relatively vague. It would be helpful to give a few concrete examples, with more literature cited, where this problem can be tested experimentally.
Authors' response: We added a few examples to the corresponding paragraph.
Reviewer's comment: (Minor comment) Figure 2 depicts the collapse of a microcommunity. It would be more illustrative to draw it together with neighbouring, non-collapsing microcommunities.
Authors' response: This is now done.
Reviewer's comment: Large, multispecies communities are a dominant form of life in the microbial world, so understanding their stability is fundamentally important. Environmental challenges, invasion by non-compatible organisms or the emergence of deleterious mutations can all disrupt stable cooperations so microbial communities have to be resilient against all of these factors. Current explanations are usually based on specific defence mechanisms that respond to individual types of challenges. The paper of Venturi et al.  develops a general scenario in which the localized nature of intercellular communication is used to explain the stability of bacterial communities. In contrast to specialized, species-specific mechanisms, this explanation is supposed to be common to all communities that need to cooperate in order to survive. It is not clear from the description if this hypothesis suggests different scenarios for monospecies and multispecies communities, it appears that it is applicable to both. Also, even though quorum sensing is a central example in this paper, the hypothesis apparently does not rely on specific bacterial features, so it may be applicable to other communities and ensembles.
Authors' response: In fact, the hypothesis can be applied to any ensemble of interacting entities that communicate locally, and as a consequence, the hypothesis can be applied to both monospecies and multispecies communities, provided that the members of the community can collectively secure the functional repertoire necessary for survival.
Reviewer's comment: Finally the authors may want to explain the relation of this supposed general mechanism to the body of literature on specific defense mechanisms. Also, it is worthwhile to look at modelling approaches that embrace components of cooperation and communication. In particular, the paper by Czárán and Hoekstra (Microbial Communication, Cooperation and Cheating: Quorum Sensing Drives the Evolution of Cooperation in Bacteria ) requires special attention.
Authors' response: This is now done in the Implication and Conclusions section of the manuscript.
Reviewer's comment: As a more general remark, one should appreciate that distinction should be made between situations that are covered by weak altruism (where the altruist pays only a relative fitness cost) and those that rest on strong altruism. Only the latter requires kin selection, although for multispecies communities the latter is not a trivial mechanism to follow. Some discussion of this issue seems in order.
Authors' response: This is a loaded question and we feel that a full discussion would be beyond the scope of this work. According to our prelimiary modelling studies, certain types of cheater mutants, with the slightest fitness advantage are capable to produce a local collapse, however we feel that the evaluation of fitness in an open, heterogeneous environments may require a more careful studies.
Finally we thank all three reviewers for the useful and thought-provoking comments.
ÁK and BR are PhD students at the Biology and Informatics PhD Program of the University of Szeged, Hungary, respectively. The work at BRC Szeged was partly supported by a grant no TAMOP-4.2.2-08/1/2008-008 from the Hungarian National Office for Research and Technology (NKTH). The help of Marietta Tóth, Pázmány Péter Catholic University, is gratefully acknowledged.
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