Figure 1

Multiple alignment of the MORCs, MGHKls and some characterized GHKL domains. Jpred predicted Secondary structure is shown above the alignment. The 75% consensus shown below the alignment was derived using the following classes of amino acids: hydrophobic (h: ALICVMYFW, yellow shading); the aliphatic subset of the hydrophobic class (l: LIV, yellow shading); hydrophobic (h: ACFILMVWY, yellow shading); small (s: ACDGNPSTV, green); the tiny subset of the small class (u: GAS, green); polar (p: CDEHKNQRST, blue); alcohol subset of polar (o: ST, brown); charged subset of polar (c: DEHKR, pink); positive subset of polar (+: HKR, pink). Amino acids in red background are completely conserved. Different subgroups of MORCS are indicated at the right end of the alignment. The sequences are denoted by their gene name followed by species abbreviation and GenBank Identifier separated by underscores. The species abbreviations are: Abac: Acidobacteria bacterium; Atha: Arabidopsis thaliana; Avar: Anabaena variabilis; Bpum: Bacillus pumilus; Bsp.: Bacillus sp.; Bsp.: Bradyrhizobium sp.; Btau: Bos taurus; Buni: Bacteroides uniformis; Bwei: Bacillus weihenstephanensis; Cbar: Clostridium bartlettii; Cbot: Clostridium botulinum; Ccre: Caulobacter crescentus; Cele: Caenorhabditis elegans; Cjej: Campylobacter jejuni; Clit: Congregibacter litoralis; Cpar: Cryptosporidium parvum; Drer: Danio rerio; Fnuc: Fusobacterium nucleatum; Fpsy: Flavobacterium psychrophilum; Glov: Geobacter lovleyi; Gthe: Geobacillus thermodenitrificans; Hsap: Homo sapiens; Jsp.: Janibacter sp.; Jsp.: Jannaschia sp.; Lbla: Leeuwenhoekiella blandensis; Lwel: Listeria welshimeri; Mace: Methanosarcina acetivorans; Mpen: Mycoplasma penetrans; Msp.: Marinobacter sp.; Nsp.: Nitrobacter sp.; Nvec: Nematostella vectensis; Oihe: Oceanobacillus iheyensis; Pcry: Psychrobacter cryohalolentis; Pflu: Pseudomonas fluorescens; Pmar: Prochlorococcus marinus; Pput: Pseudomonas putida; Pvib: Prosthecochloris vibrioformis; Retl: Rhizobium etli; Rpal: Rhodopseudomonas palustris; Rsol: Ralstonia solanacearum; Save: Streptomyces avermitilis; Ssp.: Sulfurovum sp.; Stro: Salinispora tropica; Tcru: Thiomicrospira crunogena; Tden: Treponema denticola; Vcho: Vibrio cholerae